Detection of Genetically Modified Organisms - Edvotek 121 and inserted into a plasmid

and inserted into a plasmid vector

White Light Box (optional - use if staining with FlashBlue™)

TBE Electrophoresis Buffer powder

(6) 4-20% Tris-Glycine-SDS Precast Polyacrylamide Gel (PAGE)

incubation oven (optional)

Detection of Genetically Modified Organisms - Edvotek 121 and inserted into a plasmidFor centuries, humans have used selective breeding and conventional hybridization to produce desirable qualities and to increase crop yields. Today, scientists use genetic engineering to directly manipulate the DNA, quickly producing these desirable traits. In this Edvotek experiment, students will use agarose gel electrophoresis to explore the molecular methods used by scientists to identify genetically modified organisms. No thermal cycler is